Kinase activity profiling with PamChip® peptide microarrays can be used to study signal transduction in lysates of cell lines and clinical samples. Peptides on the PamChip® arrays represent phospho-sites in the human proteome. Phosphorylation of peptides by tyrosine (PTK) or serine-threonine (STK) kinases that are active in a sample is detected in the kinase activity profiling assay.
PamGene is developing tools (“PamApps” for our proprieatary BioNavigator software) to analyse which putative “upstream” kinases, phosphorylate peptides on the PamChip® microarray using PTM databases such as HPRD, PhosphoSitePlus or data bases with in-silico predictions of which PhosphoNET contains predictions for a relatively large number of kinases.
Some of these tools are highlighted in a Poster presented at the at the BioSB Conference (May 20-21, 2015, in Lunteren, The Netherlands).