Get ‘Kinome’ insights of your biological model


Measuring kinase activity allows insights in cellular signaling, pathway elucidation, target interaction and the ability to discover biomarkers.

Get ‘Kinome’ insights of your biological model


Measuring kinase activity allows insights in cellular signaling, pathway elucidation, target interaction and the ability to discover biomarkers.

Kinases are the most intensively studied protein targets and are the basis of numerous types of therapies. However, the traditional approaches study the abundance of proteins rather than their activity. This results in a knowledge gap on how cell signaling really works and a partial understanding of your drug.

Building on 15 years of experience, PamGene has developed a unique technology making real-time measurement and understanding of kinase activity possible. Our functional phosphoproteomic assays bring on a new dimension and additional information about how cell signaling works in various disease areas.

Kinases are the most intensively studied protein targets and are the basis of numerous types of therapies. However, the traditional approaches study the abundance of proteins rather than their activity. This results in a knowledge gap on how cell signaling really works and a partial understanding of your drug.

Building on 15 years of experience, PamGene has developed a unique technology making real-time measurement and understanding of kinase activity possible. Our functional phosphoproteomic assays bring on a new dimension and additional information about how cell signaling works in various disease areas.

Applications

Pathway elucidation

  • Dive deeper into relevant pathways in cancer, immune signaling, metabolic, central nervous system or cardiovascular diseases
  • Elucidate pathways in mutants, knockouts, or as a result of in vitro treatments
  • Deduce pathways altered in drug responses (like resistance)
  • Capitalize on a broad range of pathways using without depending on specific antibodies

Biomarker discovery

On-chip pharmacology capabilities lend a new approach to discovering therapy-predictive, disease specific and prognostic or pharmacodynamic biomarkers.

Biochemical characterization

Kinase enzymology, substrate identification for novel, mutated or post-translationally modified kinases.

Diseases Model Characterization

Comparative analysis of your diseases model (in vitro or in vivo and human or rodent) with respect to healthy or wild type counterpart to gain disease specific kinome or signal transduction related information

Target discovery

Discover novel drug targets to guide drug development, where no prior target information is required.

Target interaction

Find the mechanism of action of small molecule or develop (surrogate) PD-markers.

Technology

 

In order to study kinases in biological systems, we have developed a unique method to understand kinase activity in diverse disease areas and states. Our sensitive platform uses lysates obtained without the need for complex sample preparation steps from only a few thousand cells or small amounts (0.5-5 𝜇g protein) from animal, or human sources (tissue or cells) to determine the real-time activity of all kinases present in these lysates. In addition, clinical samples can be used since our technology only needs 10.000 cells (or 1 mm3 of biopsy material) as input, compared to 1×108 cells for mass spectrometry-based proteomic assays.

This is accomplished by actively incubating the lysates across 196 to 144 tyrosine (PTK) or serine/threonine kinase (STK) peptide substrates immobilized on the PamChip microarray platform. Our 3D microarrays are spotted with peptides (i.e. phosphosites) that represent kinase targets. Kinases present in the lysates will phosphorylate the peptide substrates which are detected using fluorescently labelled antibodies. The phosphosites on the PamChips are human-derived and there is significant overlap with other organisms including rodents. PamChip microarrays are processed on the fully automated PamStation.

Based on current online knowledge, we compiled a comprehensive, integrated database (DB) of potential kinases that are linked to the peptides on the PamChips. This corresponds to ~350 unique kinases in literature, covering the majority of the kinome.

Technology

 

In order to study kinases in biological systems, we have developed a unique method to understand kinase activity in diverse disease areas and states. Our sensitive platform uses lysates obtained without the need for complex sample preparation steps from only a few thousand cells or small amounts (0.5-5 𝜇g protein) from animal, or human sources (tissue or cells) to determine the real-time activity of all kinases present in these lysates. In addition, clinical samples can be used since our technology only needs 10.000 cells (or 1 mm3 of biopsy material) as input, compared to 1×108 cells for mass spectrometry-based proteomic assays.

This is accomplished by actively incubating the lysates across 196 to 144 tyrosine (PTK) or serine/threonine kinase (STK) peptide substrates immobilized on the PamChip microarray platform. Our 3D microarrays are spotted with peptides (i.e. phosphosites) that represent kinase targets. Kinases present in the lysates will phosphorylate the peptide substrates which are detected using fluorescently labelled antibodies. The phosphosites on the PamChips are human-derived and there is significant overlap with other organisms including rodents. PamChip microarrays are processed on the fully automated PamStation.

Based on current online knowledge, we compiled a comprehensive, integrated database (DB) of potential kinases that are linked to the peptides on the PamChips. This corresponds to ~350 unique kinases in literature, covering the majority of the kinome.

Products and services

 

We offer Contract Research Services tailored to your wishes, needs and research questions. It is also possible to obtain your own PamStation. The PamGene team will support you in making the PamStation an integral part of your research facility’s departments by providing in-house training and support.

Products and services

 

We offer Contract Research Services tailored to your wishes, needs and research questions. It is also possible to obtain your own PamStation. The PamGene team will support you in making the PamStation an integral part of your research facility’s departments by providing in-house training and support.

Scientific background

Inactive immune pathways in triple negative breast cancers that showed resistance to neoadjuvant chemotherapy as inferred from kinase activity profiles.

Sawada, T. et al.
Oncotarget 9, 34229–34239 (2018).

Publication

Intratumoral spatial heterogeneity of BTK kinomic activity dictates distinct therapeutic response within a single glioblastoma tumor.

Ibrahim AN, Yamashita D, Anderson JC, Abdelrashid M, Alwakeal A et. al.
J Neurosurg. Oct 18:1-12.(2019)

Publication

Analysis of Jak2 catalytic function by peptide microarrays: the role of the JH2 domain and V617F mutation.

Sanz A, Ungureanu D, Pekkala T, Ruijtenbeek R, Touw IP, Hilhorst R, Silvennoinen O.
PLoS One. (2011) Apr 18;6(4):e18522.

Publication