Case report – Kinase Activity Profiling Services

Are you looking for thorough solutions for your questions regarding the role of kinases in your research? Measuring kinase activity provides deeper understanding of cellular signaling and target interaction.

Case report – Kinase Activity Profiling Services

Are you looking for thorough solutions for your questions regarding the role of kinases in your research? Measuring kinase activity provides deeper understanding of cellular signaling and target interaction.

PamGene is operating on the cutting edge of next generation kinase science and high-tech to support scientists and companies in discovering, studying and optimizing kinases. Building on 15 years of experience, PamGene has developed a unique technology with unprecedented sensitivity, making real-time measurement and understanding of kinase activity possible. Our technology opens up opportunities for a wide range of rapid and non-invasive applications in the areas of fundamental science, translational research and diagnostics.

CASE REPORT

PamGene is operating on the cutting edge of next generation kinase science and high-tech to support scientists and companies in discovering, studying and optimizing kinases. Building on 15 years of experience, PamGene has developed a unique technology with unprecedented sensitivity, making real-time measurement and understanding of kinase activity possible. Our technology opens up opportunities for a wide range of rapid and non-invasive applications in the areas of fundamental science, translational research and diagnostics.

As fellow scientists we are eager to learn about your challenges. The benefits our technology brings to your research are:

  • It is kinase activity-based. the direct level of inhibitor effects on kinases can be measured.
  • It has a wide multiplex coverage, the majority of the kinome is covered on our chip.
  • Our technology is sensitive, only small amounts of protein input are required
  • No specific antibodies are needed, since the quality of the data is not dependent on the specificity of phospho-antibodies
  • And to conclude we can measure kinase activity of full-length proteins from lysates of several cell lines and tissues

We’re happy to schedule an online meeting to see how our approach can benefit your research.

 

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The benefits our technology is bringing to your study

  • Kinase activity-based – Direct inhibitor effects on kinases can be measured
  • Sensitive – Only small amounts of protein input (0.5 to 5 μg per array) are required, thus making the assay more sensitive than alternative approaches e.g. MS-based phosphoproteomics.
  • Unbiased – Unlike antibody-based approaches, prior knowledge of involved kinases and targets is not required as we cover a total of 340 phosphosites.
  • No specific antibodies – The quality of the data is not dependent on the specificity of phospho-antibodies.
  • Full-length kinases – We measure kinase activity of full-length proteins from lysates of several cell lines and tissues. This is not offered by other recombinant-based kinase activity assays.