“Unravelling the PI3K-mTOR pathway“

Are you interested in gaining new publish-worthy pathway insights based on real-time cell activity?

“Unravelling the PI3K-mTOR pathway“

Are you interested in gaining new publish-worthy pathway insights based on real-time cell activity?

PamGene is operating on the cutting edge of next generation kinase science and high-tech to support scientists in unravelling the PI3K-AKT-mTOR pathway. Building on over 15 years of experience, we deliver pathway insights by measuring real-time kinase activity in signalling cascades.

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PamGene is operating on the cutting edge of next generation kinase science and high-tech to support scientists in unravelling the PI3K-AKT-mTOR pathway. Building on over 15 years of experience, we deliver pathway insights by measuring real-time kinase activity in signalling cascades.

CONTACT US

Technology

 

Kinases are the most intensively studied protein targets. We have developed a unique method to understand them. Our sensitive platform uses lysates obtained from only a few thousand cells or small amounts (2ug) of animal, xenografts or human tissues and cells to simultaneously determine the activity of all kinases present in these lysates.

This is accomplished by incubating the lysates across 196 to 144 tyrosine or serine/threonine kinase peptide substrates immobilized on the PamChip® microarray platform. Our 3D microarrays are spotted with peptides (i.e. phosphosites) that represent kinase targets. Kinases present in the lysates will phosphorylate the peptide substrates which are detected using fluorescently labelled antibodies. The phosphosites on the PamChips are human-derived and there is significant overlap with other organisms.

Based on current online knowledge, we compiled a comprehensive, integrated database (DB) of potential kinases that are linked to the peptides on the PamChips. This corresponds to ~350 unique kinases in literature, covering the majority of the kinome.

CONTACT US

Technology

 

Kinases are the most intensively studied protein targets. We have developed a unique method to understand them. Our sensitive platform uses lysates obtained from only a few thousand cells or small amounts (2ug) of animal, xenografts or human tissues and cells to simultaneously determine the activity of all kinases present in these lysates.

This is accomplished by incubating the lysates across 196 to 144 tyrosine or serine/threonine kinase peptide substrates immobilized on the PamChip® microarray platform. Our 3D microarrays are spotted with peptides (i.e. phosphosites) that represent kinase targets. Kinases present in the lysates will phosphorylate the peptide substrates which are detected using fluorescently labelled antibodies. The phosphosites on the PamChips are human-derived and there is significant overlap with other organisms.

Based on current online knowledge, we compiled a comprehensive, integrated database (DB) of potential kinases that are linked to the peptides on the PamChips. This corresponds to ~350 unique kinases in literature, covering the majority of the kinome.

The benefits our technology is bringing to your study:

  • Activity-based: Measure the activity rather than the abundance of kinases in the mTOR-AKT pathway, using cell and tissue lysates.
  • Specific coverage: The Phosphorylation of AKT and mTOR substrates can be measured on our Protein Tyrosine Kinase and Serine/Threonine Kinase assays
  • Independent of Antibody specificity: Infer pathways without requiring specific AKT pathway antibodies, in our antibody-independent assay.
  • Comprehensive and kinome-wide: Interrogate >70% kinases from our Protein Tyrosine Kinase and Serine/Threonine Kinase assays
  • Simple solutions for complex pathways: Move seamlessly and quickly from assay to biological interpretation with fast turnaround times
CONTACT US

The benefits our Kinase Inhibitor Screening technology is bringing to your study

  • Activity-based: Measure the activity rather than the abundance of kinases in the mTOR-AKT pathway, using cell and tissue lysates.
  • Specific coverage: The Phosphorylation of AKT and mTOR substrates can be measured on our Protein Tyrosine Kinase and Serine/Threonine Kinase assays
  • Independent of Antibody specificity: Infer pathways without requiring specific AKT pathway antibodies, in our antibody-independent assay.
  • Comprehensive and kinome-wide: Interrogate >70% kinases from our Protein Tyrosine Kinase and Serine/Threonine Kinase assays.
  • Simple solutions for complex pathways: Move seamlessly and quickly from assay to biological interpretation with fast turnaround times.

Applications

 

Thanks to our unique and proven technology we will provide you with reliable measurements, which can be applied in numerous of applications bringing your insights and research to the next level.

  • Canonical pathways: Link your research question to relevant cancer, immune signalling, metabolic or cardiovascular, mechanisms where AKT-MTOR are therapeutic targets.
  • Disease pathways and resistance mechanisms: Deduce pathways altered in diseases. Deduce pathways altered in acquired or innate resistance to drugs
  • Target discovery: Discover new drug targets that can guide drug development
  • Target interactions: Find the mechanism of action of small molecule inhibitors
CONTACT US

Applications

 

Thanks to our unique and proven technology we will provide you with reliable measurements, which can be applied in numerous of applications bringing your insights and research to the next level.

  • Canonical pathways: Link your research question to relevant cancer, immune signalling, metabolic or cardiovascular, mechanisms where AKT-MTOR are therapeutic targets.
  • Disease pathways and resistance mechanisms: Deduce pathways altered in diseases. Deduce pathways altered in acquired or innate resistance to drugs
  • Target discovery: Discover new drug targets that can guide drug development
  • Target interactions: Find the mechanism of action of small molecule inhibitors
CONTACT US

PI3K-mTOR pathway cases we’ve worked on

 

We have over 15 years of experience in the field of kinase science and contributed to over 100+ publications with top European, North American and Japanese Institutes.

Altered pathways – CDK4 KO in mice decreases AKT activity, implicating its involvement in insulin metabolism and signalling.

Lagarrigue S, Lopez-Mejia IC, Denechaud PD, Escoté X, Castillo-Armengol J, Jimenez V, Chavey C, Giralt A, Lai Q, Zhang L, Martinez-Carreres L, Delacuisine B, Annicotte J-S, Blanchet E, Huré S, Abella A, Tinahones FJ, Vendrell J, Dubus P, Bosch F, Kahn RC and Fajas L.
J Clin Invest. 126(1):335-348 (2016)

Publication

Disease pathways and resistance mechanisms – the mTOR pathway is activated in Breast cancer patients who are resistant to exemestane chemotherapy, and re-sensitized with 2nd line mTOR inhibitors like Everolimus.

The 42nd David A. Karnofsky Memorial Award Lecture: Understanding
Herbert M. Pinedo

Publication

PI3K-mTOR pathway cases we’ve worked on

 

We have over 15 years of experience in the field of kinase science and contributed to over 100+ publications with top European, North American and Japanese Institutes.

Altered pathways – CDK4 KO in mice decreases AKT activity, implicating its involvement in insulin metabolism and signalling.

Lagarrigue S, Lopez-Mejia IC, Denechaud PD, Escoté X, Castillo-Armengol J, Jimenez V, Chavey C, Giralt A, Lai Q, Zhang L, Martinez-Carreres L, Delacuisine B, Annicotte J-S, Blanchet E, Huré S, Abella A, Tinahones FJ, Vendrell J, Dubus P, Bosch F, Kahn RC and Fajas L.
J Clin Invest. 126(1):335-348 (2016)

Publication

Disease pathways and resistance mechanisms – the mTOR pathway is activated in Breast cancer patients who are resistant to exemestane chemotherapy, and re-sensitized with 2nd line mTOR inhibitors like Everolimus.

The 42nd David A. Karnofsky Memorial Award Lecture: Understanding
Herbert M. Pinedo

Publication

Our workflow

 

  1. Intake meeting – with our scientists to define your research goals
  2. Experimental design development – based on our extensive experience and fitted for your goals
  3. Collecting samples – by the client following our easy-to-use sample preparation protocols
  4. Process and analysis – using our unique and proven PamStation12 technique
  5. Results meeting – data will be shared and discussed with you by our scientists