Join PamGene’s Toulouse trial

Are you interested in gaining new publish-worthy signal transduction insights based on real-time kinase activity in the cell? We developed a functional 3D array making it possible to measure the activity of the majority of the kinome. This enables you to look at the bigger picture of cellular signaling and gaining full mechanistic insights.

Join PamGene’s Toulouse trial

Join our PamStation trial

We are currently introducing our technology in Toulouse, offering you the ability to measure kinase activity nearby. In a PamGene trial researchers from different fields come together to experience the technology.

The trial period duration is typically 6 to 12 months with a possibility to extend, for the researcher to prepare and validate their models. During this time we will support you scientifically and provide you with tailored reports, based on your research goals.

Reveal Cellular and Pathway Signaling

Kinases are the most intensively studied protein targets and the basis of numerous targeted therapies. However, traditional approaches study the abundance of proteins rather than their activity. This results in a knowledge gap on how cell signaling really works.

Building on almost two decades of experience, PamGene has developed a unique technology, making real-time measurement and understanding of kinase activity possible. The multiplex properties of our chip enable us to look at the bigger picture of cellular signaling. It makes you see the activity changes in pathways and provides full mechanistic insights.

Join our PamStation trial

We are currently introducing our technology in Toulouse that offers the ability to measure kinase activity. In such a PamGene trial researchers from different fields within come together to experience the technology.

This trial period duration is typically 6 to 12 months with a possibility to extend, for the researcher to prepare and validate their models. During this time we will support you scientifically and provide you with tailored reports, based on your research goals.

Reveal Cellular and Pathway Signaling

PamStation in practice

“We were looking for an additional in-house technology that would support us in improving our analysis of pathway signaling. PamGene’s unique technology, measuring all kinases and their activity in a lysate, has proven to be the best fit for this. It provides a full mechanistic insight and it is possible to combine with additional data-sets. Our hypothesis analyses have been improved and the PamStation provides us with data sets that add enormous value to ongoing research projects and collaborations which ultimately can be published in well-known scientific journals.”

Dr. Astrid Weiß, post doc. at the Biomedical Research Center Seltersberg of the Justus-Liebig-University in Giessen, Germany and scientist at the German Center for Lung Research.

Read further

Bring your research to the next level

Thanks to our unique and proven technology we will provide you with robust measurements, which can be applied in numerous of applications bringing your insights and research to the next level.

Canonical pathways: Link your research question to relevant cancer, immune or neurological cell signalling
Disease pathways and resistance mechanisms: Deduce pathways altered in diseases. Deduce pathways altered in acquired or innate resistance to drugs
Target discovery: Discover new drug targets that can guide drug development

Visit the webpage publications for a selection of studies that applied PamGene’s technology in their research.

Contact Mayke Bel for more information.

Bring your research to the next level

Thanks to our unique and proven technology we will provide you with robust measurements, which can be applied in numerous of applications bringing your insights and research to the next level.

Canonical pathways: Link your research question to relevant cancer, immune or neurological cell signalling
Disease pathways and resistance mechanisms: Deduce pathways altered in diseases. Deduce pathways altered in acquired or innate resistance to drugs
Target discovery: Discover new drug targets that can guide drug development

Visit the webpage publications for a selection of studies that applied PamGene’s technology in their research.

The benefits our technology is bringing to your study

Wide coverage – 380 Phosphosites are used to cover the majority of the Kinome.
Kinase activity-based – Direct inhibitor effects on kinases can be measured.
Sensitive – Only small amounts of protein input (0.5 to 5 μg per array) are required, thus making the assay more sensitive than alternative approaches.
No specific antibodies – The quality of the data is not dependent on the specificity of phosphoantibodies since specificity is derived from the 380 phosphosites.
Full-length kinases – We can measure kinase activity of full-length proteins from lysates of several cell lines and tissues, not offered by other recombinant-based kinase activity assays.

Technology

Kinases are the most intensively studied protein targets. We have developed a unique method to understand them. Our sensitive platform uses lysates obtained from only a few thousand cells or small amounts (2ug) of animal, xenografts or human tissues and cells to simultaneously determine the activity of all kinases present in these lysates.

This is accomplished by incubating the lysates across 196 to 144 tyrosine or serine/threonine kinase peptide substrates immobilized on the PamChip® microarray platform. Our 3D microarrays are spotted with peptides (i.e. phosphosites) that represent kinase targets. Kinases present in the lysates will phosphorylate the peptide substrates which are detected using fluorescently labelled antibodies. The phosphosites on the PamChips are human-derived and there is significant overlap with other organisms.

Based on current online knowledge, we compiled a comprehensive, integrated database (DB) of potential kinases that are linked to the peptides on the PamChips. This corresponds to ~350 unique kinases in literature, covering the majority of the kinome.