Measuring kinase activity allows insights in cellular signaling, pathway elucidation, target interaction and the ability to discover biomarkers.
Measuring kinase activity allows insights in cellular signaling, pathway elucidation, target interaction and the ability to discover biomarkers.
Kinases are the most intensively studied protein targets and are the basis of numerous types of therapies. However, the traditional approaches study the abundance of proteins rather than their activity. This results in a knowledge gap on how cell signaling really works and a partial understanding of your drug.
Building on 15 years of experience, PamGene has developed a unique technology making real-time measurement and understanding of kinase activity possible. Our functional phosphoproteomic assays bring on a new dimension and additional information about how cell signaling works in various disease areas.
Kinases are the most intensively studied protein targets and are the basis of numerous types of therapies. However, the traditional approaches study the abundance of proteins rather than their activity. This results in a knowledge gap on how cell signaling really works and a partial understanding of your drug.
Building on 15 years of experience, PamGene has developed a unique technology making real-time measurement and understanding of kinase activity possible. Our functional phosphoproteomic assays bring on a new dimension and additional information about how cell signaling works in various disease areas.
On-chip pharmacology capabilities lend a new approach to discovering therapy-predictive, disease specific and prognostic or pharmacodynamic biomarkers.
Kinase enzymology, substrate identification for novel, mutated or post-translationally modified kinases.
Comparative analysis of your diseases model (in vitro or in vivo and human or rodent) with respect to healthy or wild type counterpart to gain disease specific kinome or signal transduction related information
Discover novel drug targets to guide drug development, where no prior target information is required.
Find the mechanism of action of small molecule or develop (surrogate) PD-markers.
Learn more about this recently published review paper in ‘The current opinion in pharmacology’
In order to study kinases in biological systems, we have developed a unique method to understand kinase activity in diverse disease areas and states. Our sensitive platform uses lysates obtained without the need for complex sample preparation steps from only a few thousand cells or small amounts (0.5-5 𝜇g protein) from animal, or human sources (tissue or cells) to determine the real-time activity of all kinases present in these lysates. In addition, clinical samples can be used since our technology only needs 10.000 cells (or 1 mm3 of biopsy material) as input, compared to 1×108 cells for mass spectrometry-based proteomic assays.
This is accomplished by actively incubating the lysates across 196 to 144 tyrosine (PTK) or serine/threonine kinase (STK) peptide substrates immobilized on the PamChip microarray platform. Our 3D microarrays are spotted with peptides (i.e. phosphosites) that represent kinase targets. Kinases present in the lysates will phosphorylate the peptide substrates which are detected using fluorescently labelled antibodies. The phosphosites on the PamChips are human-derived and there is significant overlap with other organisms including rodents. PamChip microarrays are processed on the fully automated PamStation.
Based on current online knowledge, we compiled a comprehensive, integrated database (DB) of potential kinases that are linked to the peptides on the PamChips. This corresponds to ~350 unique kinases in literature, covering the majority of the kinome.
Dr. Astrid Weiß, post doc. at the Biomedical Research Center Seltersberg of the Justus-Liebig-University in Giessen, Germany and scientist at the German Center for Lung Research.
We have over 15 years of experience in the field of kinase science and contributed to over 100+ publications with top European, North American and Japanese Institutes.
To learn how our services contributed to different studies, request access to our case report.
In order to study kinases in biological systems, we have developed a unique method to understand kinase activity in diverse disease areas and states. Our sensitive platform uses lysates obtained without the need for complex sample preparation steps from only a few thousand cells or small amounts (0.5-5 𝜇g protein) from animal, or human sources (tissue or cells) to determine the real-time activity of all kinases present in these lysates. In addition, clinical samples can be used since our technology only needs 10.000 cells (or 1 mm3 of biopsy material) as input, compared to 1×108 cells for mass spectrometry-based proteomic assays.
This is accomplished by actively incubating the lysates across 196 to 144 tyrosine (PTK) or serine/threonine kinase (STK) peptide substrates immobilized on the PamChip microarray platform. Our 3D microarrays are spotted with peptides (i.e. phosphosites) that represent kinase targets. Kinases present in the lysates will phosphorylate the peptide substrates which are detected using fluorescently labelled antibodies. The phosphosites on the PamChips are human-derived and there is significant overlap with other organisms including rodents. PamChip microarrays are processed on the fully automated PamStation.
Based on current online knowledge, we compiled a comprehensive, integrated database (DB) of potential kinases that are linked to the peptides on the PamChips. This corresponds to ~350 unique kinases in literature, covering the majority of the kinome.
We offer Contract Research Services tailored to your wishes, needs and research questions. It is also possible to obtain your own PamStation. The PamGene team will support you in making the PamStation an integral part of your research facility’s departments by providing in-house training and support.
We offer Contract Research Services tailored to your wishes, needs and research questions. It is also possible to obtain your own PamStation. The PamGene team will support you in making the PamStation an integral part of your research facility’s departments by providing in-house training and support.
Khaled Alganem1, Robert McCullumsmith et al.
www.sciencedirect.com Current Opinion in Pharmacology 2022
Ibrahim AN, Yamashita D, Anderson JC, Abdelrashid M, Alwakeal A et. al.
Scientific Reports | (2022)
Isabel C. Lopez-Mejia, Andree Yeramian et al,
Molecular Oncology 17 (2023)
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