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BioNavigator is intended to be used in combination with PamChip® microarray data to analyse images and perform statistical analysis on microarray data. In this manual you’ll find background information and manuals on how to use Bionavigator for generation of your studies’ results.
The PamStation is a fully automated instrument designed for processing PamChip microarrays. In this manual you’ll find information about how to set-up and use the PamStation for your experiments.
The aim of this protocol is to prepare cell pellets from cell lines or purified cells for shipment to PamGene.
The aim of this protocol is to prepare cell lysates from cell lines or purified cells for PamChip® Kinase profiling analysis.
The aim of this protocol is to prepare cell lysates of tissue sections for use in PamChip® kinase profiling analysis.
This protocol describes a method for cutting tissue sections of biopsies or tissues for application on PamChip® kinase profiling arrays.
The aim of this protocol is the preparation of PBMC’s for shipment to PamGene. These frozen cells will be thawed, washed once and lysed internally at PamGene according to protocol 1160, for preparation of lysates of purified cells” for analysis by PamChip® Kinase and/or Tyrosine Phosphatase activity profiling.
The tools shown, are the tools we most commonly use in our data analaysis. Feel free to get in touch in case you would like to learn more.
Peptides on PamChip (PTK and STK) can be phosphorylated by a number of kinases which are represented in signaling cascades in pathways. Some basic information is shown about how we interpret the PamChip data (pathway profiles).
Data generated using PamChip assays need to be confirmed or validated. Select published examples of different approaches and methods are provided here, to illustrate possibilities for your research.
Some basic information is shown below how we interpret the PamChip data (phosphorylation profiles and predicted kinases).
The Upstream Kinase Tool is a functional scoring method that ranks the Top differential kinases. Visualization of the data on a phylogenetic tree can be useful.
A representation of major pathways and the PamChip substrates are illustrated on the Proteome map. The map represents the UniPROT Ids as provided in the Array Layout files of combined PTK and STK PamChips. Upstream Kinase Analysis (UKA) data can also be projected on Proteome maps.
Enrichr is an Online Tool for inferring Pathways and Gene Ontologies for PamChip data, both at the phosphosite level and kinase level.
g:Profiler is an Online Tool for inferring Pathways and Gene Ontologies for PamChip data, both at the phosphosite level and kinase level.
Free Online Tools for inferring Pathways and Networks for PamChip data.
Here you’ll find documents, tools and back-ground information on our most commonly used experimental designs.
Basic experiment designs are provided as a guideline to successful PamChip assays.
An overview of tools and documents related to our STK Pamchip assays.
An overview of tools and documents related to our PTK Pamchip assays.
Here you’ll find material to be used to promote your services as a PamGene core facility.
A brochure including background information on PamGene’s technology and examples of different applications. Reach out to your PamGene contact person for a tailored version.
PamGene will present a Poster at the Annual Meeting of the American Association of Cancer Reseacrh (AACR). You can download the Poster here:
