With over 20 years of expertise, PamGene delivers real-time insights into kinase activity, bridging the gap between protein abundance and functional signaling. Our multiplex KinomePro™ platform enables mechanistic understanding of drug response, pathway dynamics, and biomarker discovery for patient stratification. Unlike traditional methods, we precisely measure the activity of functional full-length kinases, not just what’s present or their mere abundance.
Our dedication to your success is underscored by our commitment to rapid turnaround times, ensuring your projects stay on schedule. Furthermore, we provide highly flexible report output, customized to seamlessly integrate with your research workflow and deliver actionable data in the format you need, truly solidifying PamGene as your ideal and responsive partner.
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Leverage our unique, activity-based KinomePro™ platform to unlock robust, functional insights across a wide array of applications, accelerating your research and deepening your understanding. Our proven technology provides the precise measurements needed to advance your studies in:
Experience a seamless journey from research question to critical insights with our tailored Contract Research Services:
Our cutting-edge technology delivers unparalleled benefits, providing you with robust, actionable insights to propel your research forward:
Direct Activity Measurement: Precisely quantify the immediate and functional effects of inhibitors directly on kinase activity, offering unparalleled insight into drug mechanisms and cellular responses.
High Sensitivity, Low Sample Input: Achieve robust and reliable results with minimal protein input (0.5 to 5 μg per array), making it ideal for precious or limited samples and more sensitive than alternative approaches like MS-based phosphoproteomics.
Unbiased Discovery: Uncover novel insights without prior assumptions. Unlike antibody-based methods, our platform comprehensively covers 340 phosphosites, enabling unbiased profiling regardless of prior knowledge of involved kinases or targets.
Antibody-Independent Approach: Ensure data quality and reproducibility, as our methodology eliminates reliance on the variable specificity and availability of phospho-antibodies.
Physiologically Relevant Full-Length Kinases: Unlike recombinant-based kinase activity assays, we measure the activity of full-length proteins directly from complex cell and tissue lysates, providing more biologically and clinically relevant insights into kinase function.
Our technology utilizes the advanced PamChip® microarray platform (PamStation®12 and PamChips PTK and STK). The 3D microarrays (PamChips PTK and STK) are precisely spotted with hundreds of human-derived phosphopeptide substrates—ranging from 144 to 196 peptides for tyrosine or serine/threonine kinases—representing key kinase targets.
During incubation, active kinases in your sample lysates phosphorylate these immobilized peptides. This phosphorylation is then detected using highly sensitive, pan-specific fluorescently labeled antibodies. The human-derived phosphosites ensure broad biological relevance, with significant overlap across various organisms, including murine rodents and other species.
To provide truly comprehensive insights, we have compiled an extensive, integrated database linking these peptides to over 380 unique kinases reported in the literature. This robust bioinformatics resource enables us to cover the majority of the kinome, delivering an unparalleled and holistic view of functional kinase activity within your samples.