Are you looking for thorough solutions for your questions regarding the role of kinases in your research? Measuring kinase activity provides deeper understanding of cellular signaling and target interaction.
Are you looking for thorough solutions for your questions regarding the role of kinases in your research? Measuring kinase activity provides deeper understanding of cellular signaling and target interaction.
Kinases are the most intensively studied protein targets and are the basis of numerous types of therapies. However, the traditional approaches study the abundance of proteins rather than their activity. This results in a knowledge gap on how cell signaling really works and a partial understanding of your drug.
Building on 15 years of experience, PamGene has developed a unique technology with unprecedented sensitivity, making real-time measurement and understanding of kinase activity possible. The multiplex properties of our chip enable us to look at the bigger picture of cellular signaling. It makes you see the activity changes in pathways, and allows the generation of a kinase-based response biomarker.
As fellow scientists we are eager to learn about your challenges. We’re happy to schedule an online meeting to see how our approach can benefit your research.
Kinases are the most intensively studied protein targets and are the basis of numerous types of therapies. However, the traditional approaches study the abundance of proteins rather than their activity. This results in a knowledge gap on how cell signaling really works and a partial understanding of your drug.
Building on 15 years of experience, PamGene has developed a unique technology with unprecedented sensitivity, making real-time measurement and understanding of kinase activity possible. The multiplex properties of our chip enable us to look at the bigger picture of cellular signaling. It makes you see the activity changes in pathways, and allows the generation of a kinase-based response biomarker.
As fellow scientists we are eager to learn about your challenges. We’re happy to schedule an online meeting to see how our approach can benefit your research.
Thanks to our unique and proven technology we will provide you with robust measurements, which can be applied in numerous of applications bringing your research and insights to the next level.
We offer Contract Research Services tailored to your needs and research questions.
Thanks to our unique and proven technology we will provide you with robust measurements, which can be applied in numerous of applications bringing your research and insights to the next level.
Learn more about this recently published review paper in ‘The current opinion in pharmacology’
We have over 15 years of experience in the field of kinase science and contributed to over 100+ publications with top European, North American and Japanese Institutes. To learn how our services contributed to different studies, request access to our case report.
Blythe NM, Muraki K, Ludlow MJ et. al.
J Biol Chem. Oct 4. pii: jbc.RA119.009167. doi: 10.1074/jbc.RA119.009167. EPub. (2019)
Chirumamilla, C. S. et al.
Methods Mol. Biol. 1930, 99–113 (2019).
Sawada T, Hilhorst R, Rangarajan S et. al.
Oncotarget. Sep 28;9(76):34229-34239. (2018)
Kinases are the most intensively studied protein targets. We have developed a unique method to understand them. Our sensitive platform uses lysates obtained from only a few thousand cells or small amounts (2ug) of animal, xenografts or human tissues and cells to simultaneously determine the activity of all kinases present in these lysates.
This is accomplished by incubating the lysates across 196 to 144 tyrosine or serine/threonine kinase peptide substrates immobilized on the PamChip® microarray platform. Our 3D microarrays are spotted with peptides (i.e. phosphosites) that represent kinase targets. Kinases present in the lysates will phosphorylate the peptide substrates which are detected using fluorescently labelled antibodies. The phosphosites on the PamChips are human-derived and there is significant overlap with other organisms.
Based on current online knowledge, we compiled a comprehensive, integrated database (DB) of potential kinases that are linked to the peptides on the PamChips. This corresponds to ~350 unique kinases in literature, covering the majority of the kinome.
Kinases are the most intensively studied protein targets. We have developed a unique method to understand them. Our sensitive platform uses lysates obtained from only a few thousand cells or small amounts (2ug) of animal, xenografts or human tissues and cells to simultaneously determine the activity of all kinases present in these lysates.
This is accomplished by incubating the lysates across 196 to 144 tyrosine or serine/threonine kinase peptide substrates immobilized on the PamChip® microarray platform. Our 3D microarrays are spotted with peptides (i.e. phosphosites) that represent kinase targets. Kinases present in the lysates will phosphorylate the peptide substrates which are detected using fluorescently labelled antibodies. The phosphosites on the PamChips are human-derived and there is significant overlap with other organisms.
Based on current online knowledge, we compiled a comprehensive, integrated database (DB) of potential kinases that are linked to the peptides on the PamChips. This corresponds to ~350 unique kinases in literature, covering the majority of the kinome.
We have over 15 years of experience in the field of kinase science and contributed to over 100+ publications with top European, North American and Japanese Institutes.
To learn how our services contributed to different studies, request access to our case report.
Blythe NM, Muraki K, Ludlow MJ, Stylianidis V, Gilbert HTJ, Evans EL, Cuthbertson K, Foster R, Swift J, Li J, Drinkhill MJ, van Nieuwenhoven FA, Porter KE, Beech DJ, Turner NA.
J Biol Chem. Oct 4. pii: jbc.RA119.009167. doi: 10.1074/jbc.RA119.009167. EPub. (2019)
Chirumamilla, C. S. et al.
Methods Mol. Biol. 1930, 99–113 (2019).
Sawada T, Hilhorst R, Rangarajan S, Yoshida M, Tanabe Y, Tamura K, Kinoshita T, Shimoyama T, van Beuningen R, Ruijtenbeek R, Tsuda H, Koizumi F.
Oncotarget. Sep 28;9(76):34229-34239. (2018)
